Aktivitas Antibakteri Ekstrak Biji Buah Kupa (Syzygium polycephalum Miq.) terhadap Escherichia coli, Staphylococcus aureus dan Candida albican
Abstract
Infections can be caused by fungi, viruses, and bacteria. Escherichia coli, Staphylococcus aureus and Candida albicans are normal bacteria in the human body and can cause disease if the human body is not balanced. Kupa is one of Indonesia's endemic plants found on the islands of Java and Kalimantan. Kupa seeds contain secondary metabolites of alkaloids, flavonoids, tannins, polyphenols, steroids, mono and sesquiterpenes which have antibacterial properties. This study aims to determine the antibacterial activity, minimum inhibitory concentration and the best inhibitory effect of Syzygium Policephalum Miq on the growth of microorganisms Staphylococcus aureus, Escherichia coli and Candida albicans. The extraction method used hexane, ethyl acetate and 70% ethanol as solvents. The method used in the antibacterial test is diffusion in agar wells. The results showed that kupa seed extract had antibacterial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The extract with the greatest inhibition was found in the ethyl acetate extract. The ethyl acetate extract had antibacterial activity against Escherichia coli, Staphylococcus aureus and Candida albicans, with MIC values of 1%, 2% and 24%, respectively.References
De Ornay, A. K., Prehananto, H., & Dewi, A.
S. S. 2017. Daya hambat pertumbuhan
Candida albicans dan daya bunuh
Candida albicans ekstrak daun
kemangi (Ocimum sanctum L.). Jurnal
Wiyata: Penelitian Sains dan
Kesehatan, 4(1), 78-83.
Harvey, R. A., Champe, P. C., Fisher, B. D.,
& Strohl, W. A. 2007. Lippincott's
illustrated reviews: Microbiology (Vol.
, p. 276). Philadelphia: Lippincott
Williams & Wilkins.
Jawets, E., Melnick, J., & Adelberg, E. 2005.
Mikrobiologi Kedokteran. jakarta:
Salemba Medika.
Ji, Y. S., Lestari, N. D., & Rinanda, T. 2012.
Uji Aktivitas antibakteri ekstrak etanol
% dan 96% kelopak bunga rosella
(Hibiscus sabdariffa) terhadap bakteri Streptococcus pyogenes secara in
vitro. Jurnal Kedokteran Syiah Kuala,
(1), 31-36.
Mujipradhana, V. N. 2018. Aktivitas
antimikroba dari ekstrak Ascidian
herdmania momus pada mikroba
patogen manusia. Pharmacon, 7(3).
Nurmalasari, T., Zahara, S., Arisanti, N.,
Mentari, P., Nurbaeti, Y., Lestari, T., &
Rahmiyani, I. 2016. Uji aktivitas
antioksidan ekstrak buah kupa
(Syzygium polycephalum) terhadap
radikal bebas dengan metode DPPH.
Jurnal Kesehatan Bakti Tunas Husada:
Jurnal Ilmu-ilmu Keperawatan, Analis
Kesehatan dan Farmasi, 16(1), 61-68.
Pelczar, M. J., & Chan, E. C. S. 1988. Dasardasar mikrobiologi. Jilid ke-1. Penerbit
UI Press. Jakarta.
Permatasari, D., Budiarti, L. Y., & Apriasari,
M. L. 2016. Efektivitas Antifungi
Ekstrak Metanol Batang Pisang Mauli
(Musa acuminata) dan Chlorhexidine
Gluconate 0,2% terhadap Candida
albicans. Dentino: Jurnal Kedokteran
Gigi, 1(1), 10-14.
Rahmiyani, I. 2018. Penetapan kadar
Flavonoid total ekstrak buah kupa
(Shyzigium polycepalum miq.)
menggunakan spektrofotometri UV-Vis.
Jurnal Kesehatan Bakti Tunas Husada:
Jurnal Ilmu-ilmu Keperawatan, Analis
Kesehatan dan Farmasi, 17(2), 487-
Renhoran, W. 2012. Aktivitas Antoksidan dan
Mikrobiologi Ekstrak Sargassum
polycystum. [Skripsi]. Departemen
Teknologi Hasil Perairan Fakultas
Perikanan Dan Ilmu Kelautan Institut
Pertanian Bogor.
Siswandono dan Bambang, S. 1995. Kimia
Medisinal. Airlangga University Press,
Surabaya.
Suryati, N., Bahar, E., & Ilmiawati, I. 2018. Uji
Efektivitas antibakteri ekstrak aloe vera
terhadap pertumbuhan Escherichia coli
secara in vitro. jurnal kesehatan
andalas, 6(3), 518-522.
Utami, P., Puspaningtyas, D. E., & Gz, S.
The Miracle of Herbs. Agro
Media.
Waluyo, L. 2007. Mikrobiologi umum edisi
revisi. UPT, Penerbit Universitas
Muhammadiyah Malang.
Wardana, A.P & Tukiran. 2016. Skrining
Fitokimia Dan Aktivitas Antioksidan
Ekstrak Kloroformtumbuhan Gowok
(Syzygium Polycephalum). Prosiding
Seminar Nasional Kimia dan
Pembelajarannya, Jurusan Kimia
FMIPA Universitas Negeri, Surabaya:
September 2016. Hal. 1-6
