Pengaruh Konsentrasi Rhizopus oligosporus terhadap Sifat Organoleptik dan Aktivitas Antioksidan Tempe Berbahan Baku Kacang Lentil
Abstract
Rhizopus oligosporus is a fungus that is often used in the manufacture of tempeh, variations in the concentration of its application can affect the organoleptic properties and antioxidant activity of the resulting product. The purpose of this study was to examine the effect of the addition of Rhizopus oligosporus on the organoleptic properties and antioxidant activity of tempeh made from lentils. Lentil beans are processed into tempeh by a fermentation process using three variations of Rhizopus oligosporus concentration. To see the effect on its physical properties, organoleptic testing was carried out on 30 panelists, including color, aroma, and texture. In addition, for the best products, the antioxidant activity was tested. The results showed that tempeh with the addition of 1.5% Rhizopus oligosporus had the most favorable physical properties by the panelists, both color 4.44 (like), aroma 4.55 (like), and texture 4.5 (like). In addition, the results of the antioxidant activity test showed that the average % inhibition values obtained were 10.26% (50 g/mL), 13.91% (100 g/mL), 18.66% (200 g/mL), 41.24% (300 g/mL), and 53.03% (400 g/mL). The conclusion from this study is the concentration of Rhizopus oligosporus affected the organoleptic properties of tempeh made from lentils, giving a concentration of 1.5% produces the most preferred physical properties by the panelists, besides that the highest antioxidant activity content is at a concentration of 300 g/mL with the average % inhibition value was 53.03%References
Astawan, M. 2011. Pangan Fungsional untuk
Kesehatan yang Optimal. Dipetik 10, 1,
, dari http://Masnafood.com.
Astuti, S. 2009. Isoflavon Kedelai dan
Potensinya sebagai Penangkap
Radikal Bebas. Jurnal Teknologi
Industri dan Hasil Pertanian. 13 (2):
-136.
Badan Standarisasi Nasional. 2009. Standar
Mutu Tempe SNI 3144-2009. Jakarta:
Badan Standarisasi Nasional.
Badan Standardisasi Nasional. 2011. Susu
Segar. SNI 01–3141–2011. Jakarta
(ID): Badan Standardisasi Nasional
Badan Standarisasi Nasional. 2012. Standar
Mutu Tempe SNI 3144-2009. Jakarta:
Badan Standarisasi Nasional.
Endang, H. 2014. ANALISIS FITOKIMIA.
Jakarta : EGC
Green, R.J. 2004. Antioxidant activity of
peanut plant Tissues. North caroline
state university departemen of food
science, Raleigh.
Goldberg I. 1994. Functional Food. New York
: Champan & Hall.
Han, I.H. 2005. Oligosaccharide reduction,
protein digestibilityimprovement,
antioxidant activity determination and
phenoliccompounds identification in.
Herman & Karmini, M., 1999. The
Development ofTempe Technology. In
J. Agranoff, ed. TheComplete
Handbook of Tempe. Singapura:
TheAmerican Soybean Association, pp.
–92.
Khazaei H, Subedi M, Nickerson, artinez CVillauengga, Frias J , Vandenberg A.
Seed Protein of Lentils: Current
Status, Progress, and Food
Applications.
Kustyawati. 2009. Kajian Peran Yeast Dalam
Pembuatan Tempe. Lampung :
Universitas Lampung.
Laksono A, Marniza, Rosalina Y. 2019.
Karakteristik Mutu Tempe Keledai
Lokal varietas Anjasmoro dengan
Variasi Lama Perebusan dan
Penggunaan Jenis Pengemas.
Bengkulu: UNIB.
DOI:10.31186/j.agroind.9.1.8-18.
pISSN: 20885369. eISSN: 26139952
Mardiani, Royyan, Maryam, Rohmatun, N.
Analisis Makronutrien dan Kadar
Glukomanan pada Tepung Iles-Iles
(Amorphophallus variabilis BI) di Kajar
Kudus. Di dalam : prosiding HEFA
(health events for all), 1 Agustus 2018.
Kudus : Lembaga Penelitian dan
Pengabdian masyarakat STIKes
Cendikia Utama Kudus. hlm 258 – 266.
Mufidah I. 2018. Analisis Perbedaan Jenis
Pembungkus Terhadap Kadar
Proksimat dan Daya Terima Tempe Biji
Lamtoro (Leucaena leucocephala).
Nangroe Aceh Darussalam:
Darussalam Nutrition Journal.ISSN
-8588. E-ISSN 2579-8618
Muthmainna, Sabang SM, Supriadi. 2016.
Pengaruh Waktu Fermentasi Terhadap
Kadar Protein dari Tempe Biji BUah
Lamtoro Gung (Leucaena
leucocephala). Palu. Univercity of
Tadukalo. ISSN 2302-6030
Profir AG, Vizireanu C. 2013. Sensorial
analysisof a functional beverage based
on vegetables juice. Acta Biol Szeged
(2):145-8
Rahayu K, Kuswanto, Sudarmaji S. 1989.
Mikrobiologi pangan PAU pangan dan
Gizi. Yogyakarta: Universitas Gajah
Mada.
Rehman Salariya AM (2005) The effects of
hydrothermalprocessing on
antinutrients, protein and starch
digestibility of food legumes. Int J Food
Sci Technol 40:695–70.
Ristia P. 2014. Meningkatkan hasil belajar
peserta didik kelas IVB melalui metode
galery walk subtema manfaat makanan
sehat dan bergizi di SD Negri 2
TinggarJaya. Purwekerto : Universitas
Muhammadiyah Purwekerto.
Sarwono B. 2008. Membuat Tempe dan
Oncom. Jakarta: Penebar Swadaya.
Saskatoon. 2010. Lentil Production Manual:
Saskacthewan Pulse growers.
Sopandi T dan Wardah. 2014. Mikrobiologi
Pangan – Teori dan Praktek.
Yogyakarta : ANDI.
Suprapti L. 2003. Pembuatan Tempe.
Yogyakarta: Kanisius.
Suwarto, A.T.C., 2011. Kinetika perubahan
asam fitat pada tempe selama proses
pemanasan. Institut Pertanian Bogor.
Tristantini, Dewi dkk. 2016. Pengujian
Aktivitas Antioksidan Menggunakan
Metode DPPH pada Daun Tanjung
(Mimusops elengi L). Yogyakarta:
Pengembangan Teknologi Kimia untuk
Pengolahan Sumber Daya Alam
Indonesia.
Widowati S, Yaniar, ME Christina & R
Holinesti. 2004. Analisis kerusakan
produk tempe kedelai (Thesis). Bogor:
IPB
Wilson, S., Blaschek, K. & de Meja, E., 2005.
Allergenic protein in soybean:
processing and reduction of P34
allergenicity. Nutr Rev, 63(2), pp.47–58
Winanti R. Bintari S H, Mustikaningtyas D .
Studi Higienitas Produk Tempe
Berdasarkan Perbedaan Metode
Inokulasi . Semarang : Unnes Journal
of Life Science. ISSN 2252-6277.
Youngson R, 2005. Antioksidant. Manfaat
Vitamin C dan E bagi kesehatan
Jakarta : Arcan