Aktivitas Antioksidan Serta Kandungan Senyawa Golongan Fenol Dari Fraksi Daun Puspa (Schima wallichii (DC.) Korth)
Keywords:
Schima wallichii (DC.) Korth, antioxidant, total phenol, total flavonoidAbstract
Puspa leaves (Schima wallichii (DC.) Korth) possess potential applications in herbal therapy. The
methanol extract of puspa leaves exhibits significant antioxidant activity. Phenolic chemicals
substantially enhance antioxidant activity.The objective of this research is to assess the antioxidant
activity of the puspa leaf fraction and to evaluate its phenolic component concentration. The liquid-liquid
extraction technique performed the fractionation using the solvents n-hexane, ethyl acetate, and
distilled water. We used the colorimetric technique with a UV-Vis spectrophotometer to quantify the total
phenolic and flavonoid content. The research findings indicated that the ethyl acetate fraction exhibited
the most antioxidant activity, with an IC50 of 6.793 ppm, while the n-hexane fraction demonstrated the
lowest activity, with an IC50 of 308.121 ppm. The ethyl acetate fraction exhibits the highest quantities
of total phenols and flavonoids, measuring 30.22 mg GAE/g and 2.13 mg QE/g, respectively.
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