Analisis Mekanisme Antihiperlipidemia Pada Senyawa Tanaman Urang Aring (Eclipta prostrata) Melalui In Silico
Abstract
Hiperlipidemia merupakan gangguan metabolisme lipid yang ditandai peningkatan kadar kolesterol dan trigliserida serta menjadi faktor risiko utama penyakit kardiovaskular. Senyawa bahan alam dengan mekanisme multi-target berpotensi menjadi kandidat terapi alternatif. Penelitian ini bertujuan menganalisis mekanisme antihiperlipidemia senyawa aktif Eclipta prostrata menggunakan pendekatan in silico. Penelitian dilakukan melalui identifikasi metabolit sekunder, screening ADME, prediksi target protein, network pharmacology, molecular docking, dan molecular dynamics. Sebanyak 53 senyawa dianalisis dan 40 memenuhi parameter ADME. Analisis menunjukkan AKT1 merupakan target utama yang berhubungan dengan jalur PI3K/AKT. Docking menunjukkan ligand+h (−9,98 kcal/mol), stigmasterol (−9,75 kcal/mol), dan verazine (−9,50 kcal/mol) memiliki afinitas tinggi. Molecular dynamics menunjukkan interaksi protein dan ligan stabil selama simulasi. Stigmasterol diprediksi sebagai kandidat antihiperlipidemia paling potensial dari Eclipta prostrata.
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