Aktivitas Antibakteri Kitosan dari Cangkang Lobster Air Tawar (Cherax quadricarinatus) terhadap Staphylococcus aureu
Abstract
Freshwater lobster shells contain chitin which can be used as an ingredient for making chitosan. Chitosan is a polysaccharide that has the potential to inhibit bacterial growth. Objective: To study the activity of chitosan from freshwater crayfish shells as an antibacterial against Staphylococcus aureus. Methods: This research was conducted by isolating chitosan from freshwater crayfish shells through four stages, namely deproteination, demineralization, depigmentation, and deacetylation. The chitosan formed was analyzed using an FTIR Spectrophotometer. The chitosan from the analysis was then tested for its activity against Staphylococcus aureus with the activity equivalence test method using Tetracycline HCl as a comparison. Results: The yields obtained from refining chitin into chitosan include deproteination (94.12%), demineralization (60.85%), depigmentation (51.35%), deacetylation (18.82%). The results of the characteristics of the FTIR spectrophotometer showed the Deacetylation Degree (DD) of chitosan based on the baseline method of 63.36%. Based on the results of testing the activity of chitosan from crayfish shells, around the hole there is a clear zone ranging from a concentration of 30% to 100% which indicates that chitosan from crayfish shells has an antibacterial inhibitory power at the smallest concentration at 30% and the minimum inhibitory concentration is 29%. The concentration of chitosan 29% w/v can inhibit the growth of Staphylococcus aureus bacteria. In the equivalence test of freshwater crayfish shell chitosan activity, a concentration of 29% w/v of chitosan from freshwater crayfish shells had antibacterial activity against Staphylococcus aureus which was equivalent to the standard concentration of Tetracycline HCl = 21.9796 µg/mL. Conclusion: Chitosan from freshwater crayfish shells has antibacterial activity against Staphylococcus aureus at a minimum concentration of 29%.References
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