Skrining Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Jeringau dan Brotowali terhadap Streptococcus pyogenes

Abstract

Jeringau (Acorus calamus L.) and brotowali (Tinospora crispa L. Miers) are plants that empirically used to treat several diseases, especially those caused by bacteria. Empirical data shows the herba of jeringau can be used to treat scabies infection. Brotowali stems which have a bitter taste are known to have many benefits, including as an antipyretic, analgesic, antiparasitic, and antiseptic. Objective: This study aimed to determine the class of active compounds contained in the jeringau and brotowali and to determine the antibacterial activity of the extracts of jeringau herbs and brotowali stems against Streptococcus pyogenes. Methods: Phytochemical screening included alkaloid, tannin, flavonoid, polyphenol and saponin+ test based on the Harborne method and antibacterial activity test of jeringau herbs and brotowali stems extracts with Kirby-Bauer disc diffusion method against Streptococcus pyogenes. Results: The results showed that the jeringau herb contained alkaloids, polyphenols, and flavonoids with antibacterial activity values at a level of 2400 mg/mL of 4.26mm. While brotowali stems contain alkaloids, tannins, polyphenols, and flavonoids with antibacterial activity values at a level of 2400 mg/mL of 3.77mm. Conclusion: The ethanol extracts of jeringau herbs and brotowali stem had antibacterial activity against Streptococcus pyogenes at concentration of 2400mg/mL.