UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN RAMANIA (Bouea macrophylla Griffith) TERHADAP BAKTERI Staphylococcus aureus
DOI:
https://doi.org/10.36465/jop.v6i3.1199Keywords:
Antibacterial, Ethanol 96%, Romania leaf (Bouea macrophylla Griffith), Staphylococcus aureusAbstract
The ramania plant (Bouea macrophylla Griffith) is a species of the Anacardiaceae tribe, with different names. Ramania plants (Bouea macrophylla Griffith) are still very limited in their use, namely only as fruits. Ramania leaves (Bouea macrophylla Griffith) empirically have the potential to control the wound healing process. One of the bacteria that can be inhibited by ramania leaves (Bouea macrophylla Griffith) is Staphylococcus aureus. The aim is to determine the results of phytochemical screening of 96% ethanol maceration extract of Ramania leaves (Bouea macrophylla Griffith) and the diameter of the inhibition zone of 96% ethanol extract of Ramania leaves (Bouea macrophylla Griffith) against Staphylococcus aureus bacteria by disc method. 96% ethanol extract of ramania leaves (Bouea macrophylla Griffith) by maceration extraction method with 96% ethanol solvent and extract yield of 8.54%. The 96% ethanol extract of Ramania (Bouea macrophylla Griffith) leaves positively contains alkoloids, flavonoids, streoids, saponins and tannins. Antibacterial activity test was performed with 4 concentration series, i.e. 5%; 10%; 15% and 20%, positive control chloramphenicol 30μg/disc, and negative control DMSO 0.1%. The results of the 96% ethanol ramania leaf (Bouea macrophylla Griffith) antibacterial activity test showed that the 5%, 10%, and 15% concentrations were included in the weak category, with inhibition zones of 1.925 mm, 3.355 mm, and 4.737 mm, respectively, while the 20% concentration was included in the moderate category with an inhibition zone of 5.775 mm. From the results of the study can show that the ethanol extract of 96% ramania leaves (Bouea macrophylla Griffith) has antibacterial activity against Staphylococcus aureus bacteria.
References
Arwita, D. (2013). Analisis Kandungan Metabolit Sekunder Pada Beberapa Koleksi Gandria (Bouea Sp.) yang Berasal dari Sumatera, Jawa, Ambon, dan Kalimantan [Universitas Negeri Medan]. http://digilib.unimed.ac.id/11852/
Conitaty, Y., Fitriyanti, F., & Hasymi, L. F. (2022). Uji Efektivitas Antibakteri Ekstrak Metanol Daun Ramania (Bouea macrophylla Griffith) Terhadap Bakteri Staphylococcus aureus. Pharmacoscript, 5(2), 212–224. https://doi.org/10.55724/jbiofartrop.v5i1.370
Kopon, A. M., Baunsele, A. B., & Boelan, E. G. (2020). Skrining Senyawa Metabolit Sekunder Ekstrak Metanol Biji Alpukat (Persea Americana Mill.) Asal Pulau Timor. Akta Kimia Indonesia, 5(1), 43. https://doi.org/10.12962/j25493736.v5i1.6709
Manik, D. F., Hertiani, T., & Anshory, H. (2016). Analisis Korelasi antara Kadar Flavonoid dengan Aktivitas Antibakteri Ekstrak Etanol dan Fraksi-fraksi Daun Kersen (Muntingia calabura L.) Terhadap Staphylococcus aureus. Khazanah: Jurnal Mahasiswa, 6(2), 1–11. https://doi.org/10.20885/khazanah.vol6.iss2.art1
Maradona, D. (2015). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Durian (Durio zibethinus L), Daun Lengkeng (Dimocarpus longan Lour), dan Daun Rambutan (Nephelium lappaceum L), Terhadap Bakteri Staphylococcus aureus ATCC 25925 dan Escherichia coli ATCC 25922 [UIN Syarif Hidayatullah Jakarta]. https://repository.uinjkt.ac.id/dspace/handle/123456789/25924 Marjoni, R. (2016). Dasar-Dasar Fitokimia. CV. Trans Info Media.
Martiningsih, M. (2016). Skrining Fitokimia dan Uji Aktivitas AntioksidanEkstrak Etanol Daun Matoa (Pometia pinnata) dengan Metode DPPH. Prosiding Seminar Nasional MIPA. Universitas Pendidikan Ganesha. https://ejournal.undiksha.ac.id/index.php/semnasmipa/article/view/10220
Muliyawan, R., Taufiqurrahman, I., & Edyson. (2018). Perbedaan Total Flavonoid Antara Metode Pengeringan Alami dan Pengeringan Buatan Pada Ekstrak Daun Ramania (Boueamacrophylla Griffith). Dentin, II(Vol 2, No 1 (2018)), 97–102. https://ppjp.ulm.ac.id/journals/index.php/dnt/article/view/417
Roni, A., Sayyidatunnisa, Z., & Budiana, W. (2019). Uji Aktivitas Antibakteri Tumbuhan Gandaria (Bouea macrophylla Griff) Terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Farmagazine, 6(1), 19. https://doi.org/10.47653/farm.v6i1.126
Susanty, S., & Bachmid, F. (2016). Perbandingan Metode Ekstraksi Maserasi dan Refluks Terhadao Kadar Fenolik dari Ekstrak Tongkol Jangung (Zea mays L.). Jurnal Konversi, 5(2), 87. https://doi.org/10.24853/konversi.5.2.87-92
Warnida, H., Mustika, D., Supomo, S., & Sukawaty, Y. (2018). Efektivitas Ekstrak Etanol Daun Mahang (Macaranga triloba) Sebagai Obat Anti Jerawat. Jurnal Penelitian Ekosistem Dipterokarpa, 4(1), 9–18. http://ejournal.forda-mof.org/ejournallitbang/index.php/JPED/article/view/4696/4266
Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania momus dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon, 10(1), 706. https://doi.org/10.35799/pha.10.2021.32758.
Zeniusa, P., Ramadhian, M. R., Nasution, S. H., & Karima, N. (2019). Uji Daya Hambat Ekstrak Etanol Teh Hijau Terhadap Escherichia coli Secara In Vitro. Jurnal Majority, 8(2), 136–143. https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/2461
