FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN LOTION EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lamk) DENGAN METODE DPPH (2,2-diphenyl-1-picrylhydrazil)

Abstract

One of the plants used as medicinal plants is Moringa (Moringa oleifera Lamk) which contains flavonoid compounds that are useful for antioxidants. The purpose of this study was to determine the stability of the lotion preparation of Moringa leaf ethanol extract and whether the preparation of Moringa leaf ethanol extract lotion had the potential as an antioxidant. Methods: Moringa leaf simplicia was extracted by maceration method using 70% ethanol as solvent. The extract was formulated in lotion preparations with concentrations of 0.8%, 1.6% and 2.4% then tested for stability using the cycling test method for 6 cycles with organoleptic testing parameters, pH, homogeneity, emulsion type, viscosity,, and flow properties. Antioxidant activity test with DPPH method using UV-Vis Spectrophotometry. Measurement of antioxidant activity was determined based on % inhibition and IC₅₀ value. Results and Conclusions: Moringa leaf ethanol extract lotion with a concentration of 0.8%; 1.6%; and 2.4% had stability on organoleptic test results, homogeneity of pH test, spreadability, emulsion type, and stable flow properties in cycle 6 of all test samples, base, and positive control still had the same flow properties as cycle 0 which was unstable only viscosity. The lotion has the the potential as an antioxidant which is expressed in % inhibition and IC₅₀ value. The results of the antioxidant activity test of the ethanol extract of Moringa leaf lotion with % inhibition values of formula I ranged from 23.89% - 25.25%, formula II 26.73% - 28.21%, formula III 35.26% - 36.63% and IC₅₀ values 562.13 ppm, 491.58 ppm, and 357.86 ppm. Moringa leaf ethanol extract lotion with a concentration of 0.8%; 1.6% and 2.4% have weak antioxidant activity but still have weak antioxidant potential.

Keywords: Moringa Leaf, Antioxidant, DPPH, Lotion, Cycling test.