Etlingera elatior FLOWER OIL ANALYSIS AND ITS PROTECTION LEVEL TEST IN MOSQUITO REPELLENT LOTION

Abstract

Etlingera elatior flowers contains flavonoids, terpenoids, saponins, and tannins. This study aims to determine
the content of oil compounds from Etlingera elatior flowers in n-hexan extract using the GC-MS method and to
find out their protective level towards Anopheles mosquitoes compared to the mosquito repellent lotions on the
markets. Extraction was carried out by maceration method using n-hexane solvent and it was purified using
96% ethanol. The analysis oil was carried out by compound derivatization method from Etlingera elatior
flower’s oil through transesterification using sodium methanolic and analyzed using GC-MS. The analysis
showed that Etlingera elatior flower’s oil contained 22 types of compounds and five of them had % AUC values
between 9 - 22.16% consisting of 9,12-octadecadienoyl chloride, 9-hexadecanoic methyl ester, octadecanoic
methyl ester, 9-octadecenoic tetradecil ester, and dodecanoic methyl ester. The results of the effectiveness test in
the form of anti-mosquito lotion showed that the mosquito repellent lotion of Etlingera elatior flower F3 (7.5%)
had a significant difference with the mosquito repellent lotion that had been sold on the market