PENGARUH KUANTITAS BAWANG PUTIH TUNGGAL (Allium sativum, L.) PADA FERMENTASI MADU TERHADAP DAYA HAMBAT BAKTERI

Aan Kunaedi

Abstract


Bacteria are one of the pathogenic microorganisms that cause infection. Honey has been used in ancient times as a topical antibacterial for the treatment of surface infections, especially open wounds. Allicin is the active substance in garlic and has antibacterial properties. The purpose of this study was to determine whether the fermented pure honey and single garlic had an inhibitory effect on Esherichia coli bacteria, in addition to knowing whether variations in the use of onions and the length of fermentation time affected the inhibitory activity of Esherichia coli bacteria. The inhibition test was carried out using the paper disc diffusion method. The sample used was fermented pure honey with a single garlic ratio of 1:2, 1:1 and 2:1 at the 2nd, 3rd and 4th weeks. Positive control using amoxicillin injection concentration of 0.01%, negative control using sterile distilled water and pure honey as a comparison. The results showed that fermented honey with a ratio of 1:2, 1:1 and 2:1 had a strong category of inhibitory activity at weeks 2, 3 and 4. Fermented honey has inhibitory activity against Esherichia coli bacteria and there is an effect of comparison ratio and length of fermentation time on inhibitory activity against Esherichia coli bacteria.

Keywords


Single Garlic (Allium sativum, L.), Pure Honey, Paper Disc Diffusion, Esherichia coli.

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References


Amini, I. R. (2021). Zona Hambat Ekstrak Bawang Putih Tunggal (Allium sativum) Terhadap Streptococcus mutans ATCC® 21752TM (In Vitro). SKRIPSI.

Andam Dewi, M., Emran Kartasasmita, R., Singgih Wibowo, M., & Yani, A. (2017). UJI AKTIVITAS ANTIBAKTERI BEBERAPA MADU ASLI LEBAH ASAL INDONESIA TERHADAP Staphylococcus aureus dan Escherichia coli. Kartika : Jurnal Ilmiah Farmasi, 5(1), 27–30.

Damayanti, E. (2018). DAYA HAMBAT MADU HUTAN PEDALAMAN ULUBONGKA TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Streptococcus pneumoniae. In Skripsi. Universitas Muhammadiyah Semarang. Fakultas Keperawatan. (Vol. 53, Issue 9, pp. 1689–1699).

Dewi, M. A., Kartasasmita, R. E., & Wibowo, M. S. (2017). UJI AKTIVITAS ANTIBAKTERI BEBERAPA MADU ASLI LEBAH ASAL INDONESIA TERHADAP Staphylococcus aureus dan Escherichia coli. Kartika Jurnal Ilmiah Farmasi, 5(1), 27–30.

Diah, D. W. (2018). Madu Sebagai Terapi Komplementer. Graha Ilmu.

Fahmi, Y. I., Andriana, A., & Hidayati, D. S. (2019). UJI DAYA HAMBAT EKSTRAK BAWANG PUTIH (Allium sativum) TERHADAP BAKTERI (Staphylococcus Aureus). JURNAL KEDOKTERAN, 4(2), 82.

Fitrianingsih, S. P., Khairat, A., & Choestrina, R. (2014). Aktivitas antibakteri madu Pahit dan Madu Hitam manis terhadap Escherichia coli dan Staphylococcus aureus. Jurnal Farmasi Galenika, 1(2), 32–37.

Gasyiya, N. W. A. (2018). pengaruh lama fermentasi terhadap beberapa komponen mutu solo black garlic dari bawang putih (allium sativum linn) varietas lumbu hijau. Artikel Ilmiah, 2.

Haryo, B. S. R. (2020). Teknologi Fermentasi Pangan Tradisional dan Produk Olahannya.

Indrayati, S., & Diana, P. E. (2020). Uji Efektifitas Larutan Bawang Putih (Allium sativum) terhadap pertumbuhan Bakteri Staphylococcus Epidermidis. JURNAL KESEHATAN PERINTIS (Perintis’s Health Journal), 7(1), 22–31.

Jawetz, Melinick, & Aldeberg. (2008). Mikrobiologi Kedokteran (Vol. 23). EGC.

Kaligis, C. J., Nangoy, E., & Mambo, C. D. (2020). Uji Efek Anti Bakteri Madu Hutan dan Madu Hitam Terhadap Bakteri Staphylococcus aureus, Escherichia coli, dan Pseudomonas aeruginosa. E-Biomedik, 8(1), 112–119.

Karnia, I., Hamidah, S., & Thamrin, G. A. R. (2019). Pengaruh Masa Simpan Madu Kelulut (Trigona sp.) terhadap Kadar Gula Pereduksi dan Keasaman. J. Sylva Scienteae, 2(6), 1094–1099.

Libonatti, C., Soledad, V., Marina, B., Carina, L., Soledad, V., & Marina, B. (2014). Journal of Microbiology and Antimicrobials Antibacterial activity of honey: A review of honey around the world. Journal of Microbiology and Antimicrobials, 6(3), 51–56.

Moulia, M. N., Syarief, R., Iriani, E. S., Kusumaningrum, H. D., Suyatma, N. E., Penelitian, B. B., Pengembangan, D., Pertanian, P., Penelitian, B., Pertanian, P., & Pertanian, K. (2018). Antimikroba Ekstrak Bawang Putih. JURNAL PANGAN, 27(1), 55–66.

Muh. Yunus, S. S. . M. K. N., & Abbas, M. (2019). UJI DAYA HAMBAT MADU HUTAN MURNI (MEI DEPURATUM) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS. Majalah Farmasi Nasional, 16(1), 6–12.

Pratiwi, S. T. (2008). Mikrobiologi Farmasi. Erlangga.

Prestianti, I. (2017). Uji Aktivitas Ekstrak Sarang Lebah Dan Madu Hutan Dari Kolaka Terhadap Pertumbuhan Bakteri Staphylococcus aureus, Escherichia coli Dan Pseudomonas Aeruginosa. Skripsi. Makassar: Jurusan Kimia Pada Fakultas Sains Dan Teknologi. UIN Alauddin Makassar, 1–94.

Prihandani, S. S. (2015). UJI DAYA ANTIBAKTERI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, Salmonella typhimurium DAN Pseudomonas aeruginosa DALAM MENINGKATKAN KEAMANAN PANGAN. Informatika Pertanian, 24(1), 53.

Radji, M. (2011). Mikrobiologi panduan mahasiswa farmasi dan kedokteran. EGC.

Ria Khoirunnisa Apriyani, S. C. (2021). UJI AKTIVITAS ANTIBAKTERI MADU HITAM PAHIT DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Esherichia coli DI LABORATORIUM ANALIS KESEHATAN POLITEKNIK PIKSI GANESHA BANDUNG. Jurnal INFOKES-Politeknik Piksi Ganesha, 5(1), 36–41.

Simpson, C. A., Geornaras, I., Yoon, Y., Scanga, J. A., Kendall, P. A., Sofos, J. N., & Dalynn Biologicals. (2014). McFarland Standard. Journal of Food Protection, 71(3), 2.

Sun, D. M., Indriarini, D., & Nurina, R. L. (2019). UJI AKTIVITAS ANTIBAKTERI LARUTAN MADU HUTAN TERHADAP PERTUMBUHAN Escherichia coli SECARA IN VITRO. Cendana

Syawalludin, R., Sakit, R., Kamino, H., & Kanan, W. (2019). KEMAMPUAN MADU HITAM DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Pseudomonas aeruginosa. Jurnal Ilmu Kedokteran Dan Kesehatan, 6(4).

Thambavita, D., Galappatthy, P., Mannapperuma, U., Jayakody, L., Cristofoletti, R., Abrahamsson, B., Groot, D. W., Langguth, P., Mehta, M., Parr, A., Polli, J. E., Shah, V. P., & Dressman, J. (2017). Biowaiver Monograph for Immediate-Release Solid Oral Dosage Forms: Amoxicillin Trihydrate. Journal of Pharmaceutical Sciences, 106(10), 2930–2945.




DOI: http://dx.doi.org/10.36465/jop.v5i2.916

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